31. T-DNA transfer and processing into the plant genome require products of which of the following genes?
A. vir A, B
B. vir G, C
C. vir D, E
D. All of these
32. Because of the large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into
A. pBR 322 based plasmid vector
B. pRK 2013
C. pRN 3
D. all of these
33. The transfer of intermediate vectors into Agrobacterium are brought about by
A. transformation
B. biparental mating
C. triparental mating
D. transduction
34. In response to the activating signal molecule, an endonuclease is produced that causes nicks in the T-DNA. It is encoded by
A. vir A
B. vir B
C. vir C
D. vir D
35. Microinjection involves
A. injection of large amount of DNA
B. injection of DNA into bigger cells
C. injection with needle having diameter greater than cell diameter
D. all of the above