Chromatography – Section 1

1. Thin layer chromatography is

A. partition chromatography
B. electrical mobility of ionic species
C. adsorption chromatography
D. none of the above

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2. In gas chromatography, the basis for separation of the components of the volatile material is the difference in

A. partition coefficients
B. conductivity
C. molecular weight
D. molarity

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3. In reverse phase chromatography, the stationary phase is made

A. non-polar
B. polar
C. either non-polar or polar
D. none of these

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4. Ion exchange chromatography is based on the

A. electrostatic attraction
B. electrical mobility of ionic species
C. adsorption chromatography
D. partition chromatography

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5. The general expression for the appearance of a solute in an effluent is (where V is the elution volume of a substance , V₀ void volume, k_D distribution constant and V_i internal water volume)

A. V = V₀+ k_DV_i 
B. V = V₀/V_i
C. V = V₀– k_DV_i
D. V/V₀= k_DV_i

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