Animal Cell Culture and Regulation – Section 1

26. The technique of organ culture may be divided on the basis of employing

A. solid medium
B. liquid medium
C. both (a) and (b)
D. semi-solid medium

27. At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via

A. oxidative pathway
B. anaerobic pathway
C. both (a) and (b)
D. none of these

28. An established cell line can be called where it has been subcultured at least

A. 70 times at an interval of 3 days between subcultures
B. 40 times at an interval of 3 days between subcultures
C. 70 times at an interval of 1 day between subcultures
D. 50 times at an interval of 3 days between subcultures

29. Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively)

A. 0.05-5 nmol of O₂/cell/h
B. 5 – 10 nmol of O₂/cell/h
C. 0.05-5 pmol of O₂/cell/h
D. 5 – 10 pmol of O₂/cell/h

30. Which of the following is not a source of energy in active muscle cells?

A. Creatine phosphate
B. ATP
C. Lactic acid
D. Glucose