Animal Cell Culture and Regulation – Section 1

11. What is the concentration of CO₂ required for culturing animal cells?

A. 2-5%
B. 1-10%
C. 10-15%
D. 15-20%

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12. Which of the following is correct?

A. ECL can be established in suspension cultures whereas it is exceptional for primary cell lines (PCL)
B. ECL and PCL can be established in suspension cultures
C. PCL can be established in suspension cultures
D. none of the above

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13. Which of the following are the metabolic products of glucose and glutamine?

A. CO₂ and NH₃
B. CO₂ and lactate
C. Lactate and ammonium
D. Lactate only

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14. To prevent the accumulation of lactate

A. low glutamine concentration is required
B. high glutamine concentration is required
C. low glucose concentration is required
D. high glucose concentration is required

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15. What are the main constituents of culture for animal cell growth?

A. Glucose and Glutamine
B. Growth factors
C. Cytokines
D. All of these

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